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. 2019 Nov 11;45(1):175–185. doi: 10.3892/ijmm.2019.4400

Figure 2.

Figure 2

Effects of hypoxia on the secretion of cytokines by HMC-1 cells. (A) HMC-1 cells were cultured under hypoxic conditions for the indicated time periods and then subjected to reverse transcription-quantitative polymerase chain reaction to detect the expression of cytokines IL-1β, IL-6, IL-15 and TNF-α. β-tubulin was used as an internal control. (B-E) Following culture under hypoxic conditions, the levels of the secreted cytokines IL-1β, IL-6, IL-15 and TNF-α in the conditioned medium from HMC-1 cells were determined by ELISA. There were 4-6 biological replicates/condition. Error bars depict the standard deviation. *P<0.05, **P<0.01, NS, not significant. IL, interleukin; TNF, tumor necrosis factor; HMC-1, human mast cells.