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. 2019 Nov 11;45(1):175–185. doi: 10.3892/ijmm.2019.4400

Figure 6.

Figure 6

Effects of conditioned medium from HMC-1 cells on the transition of HFL-1 cells from fibroblasts to myofibroblasts. (A) After treatment with nor-moxic or hypoxic conditioned medium from HMC-1 cells, the expression of α-SMA mRNA was determined by reverse transcription-quantitative polymerase chain reaction analysis. β-tubulin was used as an internal control. (B) Western blotting was performed to assess the level of α-SMA protein in HFL-1 cells cultured in normoxic or hypoxic conditioned medium from HMC-1 cells. (C) After treatment with conditioned medium from HMC-1 cells, HFL-1 cells were stained for immunofluorescence for α-SMA. Nuclei were counterstained with DAPI. There were 4-6 biological replicates/condition. Error bars represent the standard deviation. *P<0.05, **P<0.01. Bar, 50 µm. α-SMA, α-smooth muscle actin; DAPI, 4′,6-diamidino-2-phenylindole; HMC-1, human mast cells; HFL-1, human fetal lung fibroblasts.