Figure 6.

Combination treatment with cordycepin and JSH-23 inhibits the activation of NF-κB signaling and downregulates CXCR4 expression. Cells were treated with 10 µM cordycepin, 1 µM JSH-23 or their combination, and the expression levels of CXCR4 and the phosphorylation status of IκB and P65 were analyzed by western blotting. (A) Representative blots and (B) densitometry analysis in HepG2 cells. (C) Representative blot and (D) densitometry analysis in Huh7 cells. Data are presented as the mean ± SD. ^*P<0.05 vs. untreated control; and ^#P<0.05 vs. cordycepin alone. CXCR4, C-X-C chemokine receptor type 4; IκBα, NF-κB inhibitor α; p-, phosphorylated; t-, total.