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. 2019 Dec;61(6):747–754. doi: 10.1165/rcmb.2019-0006OC

Figure 1.

Figure 1.

Schematics of hybrid vectors. (A) A PB/adeno-associated virus (AAV) transposon was delivered by a single-stranded AAV (ssAAV). The transposon terminal repeats (TRs) flank the F5Tg83 promoter, driving expression of human CFTRΔR or puromycin resistance, followed by a shortened synthetic polyadenylation signal (pA) (19). (B) The piggyBac transposase driven by the F5Tg83 promoter followed by the short polyA is represented schematically in either the ssAAV or self-complementary AAV (scAAV) vector. bp = base pairs; hCFTR = human cystic fibrosis transmembrance conductance regulator; ITR = inverted terminal repeat; PB = piggyBac.