Figure 1.

Generation and characterization of ARPC2‐knockout (KO) DLD‐1 colon cancer cells. A, Using the clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein 9 (CRISPR/Cas9) system, we established ARPC2‐KO cell lines in DLD‐1 cells. Cell lines were confirmed by genomic DNA sequencing. B, Disappearance of the Arp2/3 complex was detected by western blot analysis (n = 3). C, The shape of DLD‐1 and DLD‐1‐ARPC2 KO cells was visualized through a microscope (n = 2). Scale bars, 200 μm. D, Cell migration assay was done with DLD‐1 and DLD‐1‐ARPC2 KO cells (n = 3). Scale bars, 20 μm. E, Lamellipodium formation was analyzed by confocal microscopy. Staining of cortactin (lamellipodia marker) and F‐actin shows reduction of lamellipodium formation in DLD‐1‐ARPC2‐KO cell lines (n = 2). Data represent means ± SD compared with the corresponding control, **P < .01