Figure 5.

Pimozide binding to ARPC2 delays the outset of Arp2/3 complex‐mediated actin polymerization and inhibits vinculin‐dependent Arp2/3 complex localization to focal adhesion. A, Assay was done using an actin polymerization biochemical kit. Actin polymerization was measured by pyrene fluorescence at 365 nm/405 nm (n = 3). B, Starved DLD‐1 cells were activated with FBS for the indicated times and immunoprecipitated with a vinculin antibody. Expression level of p‐ERK1/2 shows that cells are activated sufficiently by FBS. (n = 3) C, Immunoprecipitation of vinculin antibody from starved DLD‐1 cells that were treated with DMSO or pimozide (5, 10 or 20 μmol/L) for 30 min. Activation time of FBS was 5 min (n = 3). D, Confocal images of DLD‐1 cells that were treated with DMSO or pimozide (10 μmol/L) for 4 h (n = 2)