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. 2019 Nov 17;110(12):3708–3717. doi: 10.1111/cas.14221

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© 2019 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Expression of cancer stem cell (CSC) markers in SS‐2 spheres. A, Findings from qRT‐PCR analysis show that spheres formed by SS‐2 cells expressed higher levels of CD133 compared to cells cultured in adherent conditions. Expression of CD166, CD24 and CD44 mRNA did not differ significantly between the spheres and adherent SS‐2 cells. *P < .05, ^† P < .01. B, Expression of CD133 in SS‐2 cells as determined by flow cytometric analysis. More CD133+ cells were evident among spheres compared to cells cultured in adherent conditions. Representative results are shown. Gating strategy represents CD133+ cells. Right panel shows CD133 expression as mean fluorescence intensity (MFI)