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. 2019 Nov 19;110(12):3650–3662. doi: 10.1111/cas.14206

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© 2019 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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In case 12 of refractory pediatric solid tumor, ex vivo interferon (IFN)‐γ enzyme‐linked immunospot (ELISPOT) assay (A), in vitro IFN‐γ ELISPOT assay (B), and in vitro Dextramer assay (C) were carried out using PBMCs obtained after vaccination with NCCV Cocktail‐1. Each peptide‐specific spot number of KOC1, FOXM1, and KIF20A indicates the number of each peptide‐specific spot calculated by subtracting the spot number in a well of HIV peptide. When the number of IFN‐γ‐positive spots was 10 or more, it was defined that the peptide‐specific CTLs could be induced (shown by red boxes)