Figure 5.

MicroRNA (miR)‐338‐5p inhibited invasion and migration of esophageal squamous cell carcinoma (ESCC) cells in vitro by inhibiting Id‐1. A, Representative images and quantification of Transwell cell migration assay showing inhibition of invasion of ESCC (KYSE270 and T.Tn) cells by miR‐338‐5p, and the attenuation of such effects by reexpression of Id‐1. B, Wound healing assay showing the effects of miR‐338‐5p overexpression, with and without ectopic expression of Id‐1, on migration of ESCC cells. C, Transwell cell invasion assay of KYSE270 and T.Tn cells transfected with miRZip‐338‐5p, miRZip‐338‐5p in combination with shId‐1, or scrambled control (Ctrl). D, Wound healing assay comparing the migration potential of KYSE270 and T.Tn cells following miR‐338‐5p silencing with and without Id‐1 knockdown