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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Addict Biol. 2018 Jan 23;24(2):206–217. doi: 10.1111/adb.12587

Figure 2.

Figure 2

Effect of acupuncture on production of 50-kHz ultrasonic vocalizations (USVs) in methamphetamine (METH)-administered rats. (a) A customized experimental chamber to measure USVs in freely moving rats. (b) Exemplary spectrograms of 50-kHz USVs. (c) Total number of 50-kHz USVs for 40 minutes and (d) time course of 50-kHz USVs during every 10 minutes after systemic injection of METH. Data are presented as mean ± standard error of the mean. $P < 0.05 versus baseline of METH. *P < 0.05, **P < 0.01 versus METH. N value is 6–7 per group. Acu = acupuncture; METH = METH-treated rats; METH + HT7 = acupuncture at HT7 in METH-treated rats; METH + TE4 = acupuncture at TE4 in METH-treated rats