Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic Reagent (M. musculus; male and female) | C57BL/6 mice | National Institute on Aging | RRID:SCR_007317 | 16 months old |
| Biological sample (M. musculus) | Cecal slurry; CS | Starr and Saito, 2014; Steele et al., 2017 | Donor mice: 16 week old male C57BL/6 mice obtained from The Jackson Laboratory | |
| Antibody | Mouse monoclonal myosin heavy chain type I (MIgG2b) | Developmental Studies Hybridoma Bank | Cat# BA-D5; RRID: AB_2235587 |
1:100 |
| Antibody | Mouse monoclonal myosin heavy chain type IIa (MIgG1) | Developmental Studies Hybridoma Bank |
Cat# SC-71; RRID: AB_2147165 |
1:100 |
| Antibody | Mouse monoclonal myosin heavy chain type IIb (MIgM) | Developmental Studies Hybridoma Bank | Cat# BF-F3; RRID: AB_2266724 |
1:100 |
| Antibody | Goat anti-mouse IgG2b, Alexa Fluor 647 conjugated | Life Technologies- Thermo Fisher | Cat# A-21242; RRID: AB_2535811 | 1:250 |
| Antibody | Goat anti-mouse IgG1, Alexa Fluor 488 conjugated | Life Technologies- Thermo Fisher | Cat# A-21121; RRID: AB_2535764 | 1:500 |
| Antibody | Goat anti-mouse IgM, Alexa flour 555 conjugated | Life Technologies- Thermo Fisher | Cat# A-21426; RRID: AB_2535847 | 1:250 |
| Antibody | Mouse monoclonal 3-Nitrotyrosine (39B6) | Abcam | Cat# ab61392; RRID: AB_942087 | 1:3000 |
| Antibody | Goat anti-mouse IgG-HRP | Santa Cruz | Cat# SC-2005 | 1:10,000 |
| Commercial assay or kit | High sensitivity IL-6 mouse ELISA kit | eBIOSCIENCES- Thermo Fisher | Cat# BMS603HS; RRID: AB_2575654 | Sepsis 4d plasma samples diluted 5X for assay |
| Commercial assay or kit | V-PLEX multiplex assay | V-Plex Proinflammatory Panel 1 Mouse Kit, customized | Cat# K15048D | |
| Commercial assay or kit | PureLink RNA Mini Ki | Invitrogen-Thermo Fisher | Cat# 12183025 | |
| Commercial assay or kit | SuperScript III First-Strand Synthesis SuperMix | Life Technologies- Thermo Fisher | Cat# 18080400 | |
| Commercial assay or kit | TaqMan mouse IL-6 gene expression assay |
Thermo Fisher | Assay ID: Mm00446190_m1 | |
| Commercial assay or kit | TaqMan mouse TNFα gene expression assay | Thermo Fisher | Assay ID: Mm00443258_m1 | |
| Commercial assay or kit | TaqMan mouse IL-10 gene expression assay | Thermo Fisher | Assay ID: Mm01288386_m1 | |
| Commercial assay or kit |
TaqMan mouse HPRT gene expression assay |
Thermo Fisher | Assay ID: Mm03024075_m1 | |
| Commercial assay or kit | BCA protein assay kit | Thermo Fisher | Cat# 23225 | |
| Commercial assay or kit | RC DC protein assay kit II | Bio-Rad | Cat# 5000122 | |
| Commercial assay or kit |
OxyBlot Protein Oxidation Detection Kit | EMD Millipore | Cat# S7150 | |
| Chemical compound, drug | Imipenem; IPM | Primaxin IV; imipenem 500 mg stabilized in cilastatin |
NDC 0006-3516-59 | 1.5 mg/mouse |
| Chemical compound, drug |
Physiological Saline (0.9%) | Abbott Laboratories | Ref. # 04930-04-10 | |
| Software | ZEN (blue edition) imaging software | Zeiss | RRID:SCR_013672 | |
| Software | ImageJ software (version 1.46 r) | National Institutes of Health | RRID:SCR_003070 | |
| Software | Image Lab software (2017) | Bio-Rad | Cat# #1709690 | |
| Software | SAS 9.4 | SAS Institute Inc | ||
| Software | Aperio ImageScope software (12.4) | Leica | RRID:SCR_014311 | Positive-pixel algorithm |