Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | Psi | FLYB:FBgn0014870 | Flybase name:P-element somatic inhibitor | |
| Gene (Drosophila melanogaster) | tim | FLYB:FBgn0014396 | Flybase name: timeless | |
| Gene (Drosophila melanogaster) | tio | FLYB:FBgn0028979 | Flybase name: tiptop | |
| Gene (Drosophila melanogaster) | per | FLYB:FBgn0003068 | Flybase name: period | |
| Gene (Drosophila melanogaster) | cwo | FLYB:FBgn0259938 | Flybase name:clockwork orange | |
| Gene (Drosophila melanogaster) | RpL32 | FLYB:FBgn0002626 | qPCR control Flybase name:Ribosomal protein L32 |
|
| Gene (Drosophila melanogaster) | larp | FLYB:FBgn0261618 | Flybase name: La related protein |
|
| Gene (Drosophila melanogaster) | Rbp9 | FLYB:FBgn0010263 | Flybase name:RNA-binding protein 9 | |
| Gene (Drosophila melanogaster) | Dcr-2 | FBgn0034246 | Flybase name: Dicer-2 |
|
| Genetic reagent (D. melanogaster) | tim-GAL4 | Kaneko et al., 2000 | FLYB:FBtp0010385 | |
| Genetic reagent (D. melanogaster) | Pdf-GAL4 | Renn et al., 1999 | FLYB:FBtp0011844 | |
| Genetic reagent (D. melanogaster) |
Pdf-GAL80, Pdf-GAL80 | Stoleru et al., 2004 | ||
| Genetic reagent (D. melanogaster) | UAS-Dcr2 | Dietzl et al., 2007 | FLYB:FBti0100275 RRID:BDSC_24650 |
Chromosome 2 |
| Genetic reagent (D. melanogaster) | UAS-Dcr2 | Dietzl et al., 2007 | FLYB:FBti0100276 | Chromosome 3 |
| Genetic reagent (D. melanogaster) | PsiRNAi KK101882 | FLYB:FBal0231542 | ||
| Genetic reagent (D. melanogaster) | PsiRNAi GD14067 | Dietzl et al., 2007 | FLYB:FBst0457756 | |
| Genetic reagent (D. melanogaster) | UAS-Psi | Labourier et al., 2001 | ||
| Genetic reagent (D. melanogaster) | BG-LUC | Stanewsky et al., 1997 | ||
| Genetic reagent (D. melanogaster) | ptim-TIMLUC | Lamba et al., 2018 | ||
| Genetic reagent (D. melanogaster) | timHA | Rutila et al., 1998 | FLYB:FBal0143160 | |
| Genetic reagent (D. melanogaster) | tim0 | Sehgal et al., 1994 | FLYB:FBal0035778 | |
| Genetic reagent (D. melanogaster) | VIE260B | VDRC_ID: 60100 |
||
| genetic reagent (D. melanogaster) |
larpRNAi
GD8214 |
Dietzl et al., 2007 | VDRC_ID: 17366 |
|
| Genetic reagent (D. melanogaster) |
Rbp9RNAi
KK109093 |
VDRC_ID: 101412 |
||
| Genetic reagent (D. melanogaster) | w1118 | VDRC_ID: 60000 |
||
| Genetic reagent (D. melanogaster) | 40D-UAS | VDRC_ID: 60101 |
||
| Sequence-based reagent | RpL32-forward | Dubruille et al., 2009 | PCR primers | ATGCTAAGCTGTCGCACAAA |
| Sequence-based reagent | RpL32-reverse | Dubruille et al., 2009 | PCR primers | GTTCGATCCGTAACCGATGT |
| Sequence-based reagent | psi-forward | This paper | PCR primers | GGTGCCTTGAATGGGTGAT |
| Sequence-based reagent | psi-reverse | This paper | PCR primers | CGATTTATCCGGGTCCTCG |
| Sequence-based reagent | tim-M-forward | This paper | PCR primers | TGGGAATCTCGCCCGAAAC |
| Sequence-based reagent | tim-M-reverse | This paper | PCR primers | AGAAGGAGGAGAAGGAGAGAGG |
| Sequence-based reagent | tim-sc-forward | This paper | PCR primers | ACTGTGCGATGACTGGTCTG |
| Sequence-based reagent | tim-sc-reverse | This paper | PCR primers | TGCTTCAAGGAAATCTTCTG |
| Sequence- based reagent |
tim-cold-forward | This paper | PCR primers | CCTCCATGAAGTCCTCGTTCG |
| Sequence-based reagent | tim-cold-reverse | This paper | PCR primers | ATTGAGCTGGGACACCAGG |
| Sequence-based reagent | cwo-foward | This paper | PCR primers | TTCCGCTGTCCACCAACTC |
| Sequence-based reagent | cwo-reverse | This paper | PCR primers | CGATTGCTTTGCTTTACCAGCTC |
| Sequence-based reagent | cwoRA-forward | This paper | PCR primers | TCAAGTATGAGAGCGAAGCAGC |
| Sequence-based reagent | cwoRA-reverse | This paper | PCR primers | TGTCTTATTACGTCTTCCGGTGG |
| Sequence-based reagent | cwoRB-forward | This paper | PCR primers | GTATGAGAGCAAGATCCACTTTCC |
| Sequence-based reagent | cwoRB-reverse | This paper | PCR primers | GATGATCTCCGTCTTCTCGATAC |
| Sequence-based reagent | cwoRC-forward | This paper | PCR primers | GTATGAGAGCCAAGCGACCAC |
| Sequence-based reagent | cwoRC-reverse | This paper | PCR primers | CCAAATCCATCTGTCTGCCTC |
| Commercial assay or kit | Direct-zol RNA MiniPrep kit | Zymo Research | Zymo Research: R2050 | |
| Commercial assay or kit | iSCRIPT cDNA synthesis kit | Bio-RAD | Bio-RAD: 1708891 | |
| Commercial assay or kit | iTaq Universal SYBR Green Supermix | Bio-RAD | Bio-RAD: 1725121 | |
| Chemical compound, drug | D-Luciferin, Potassium Salt | Goldbio | Goldbio: LUCK-1G | |
| Chemical compound, drug | TRIzol Reagent | Invitrogen | ThermoFisher Scientific:15596026 | |
| Software, algorithm | FaasX software | Grima et al., 2002 | http://neuro-psi.cnrs.fr/spip.php?article298&lang=en | |
| Software, algorithm |
MATLAB (MathWorks) signal-processing toolbox | Levine et al., 2002 | MATLAB RRID: SCR_001622 | |
| Software, algorithm | MS Excel | RRID: SCR_016137 | ||
| Software, algorithm | GraphPad Prism version 7.0 c for Mac OS X | GraphPad Software, La Jolla, CA USA | RRID: SCR_002798 | www.graphpad.com |