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. 2019 Nov 29;14:9337–9349. doi: 10.2147/IJN.S218458

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© 2019 Du et al.

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SDS-PAGE analysis of SIV-NP recombinant protein.

Notes: (A) The expression of SIV-NP protein from soluble fraction was analyzed using 12% SDS-PAGE. MW, protein molecular markers; lane 1, bacterial lysates from pET-32a-transformed cells without IPTG; lane 2, bacterial lysates from IPTG-induced pET-32a-transformed cells; lane 3, bacterial lysates from pET-32a-SIV-NP-transformed cells without IPTG; lane 4, bacterial lysates from 0.4 mM IPTG-induced pET-32a-SIV-NP-transformed cells; lane 5, bacterial lysates from 0.8 mM IPTG-induced pET-32a-SIV-NP-transformed cells. (B) The SIV-NP protein was purified using a Ni-NTA chromatography and analyzed by 12% SDS-PAGE. MW, protein molecular markers; Lane 1–8, sequential fractions from the Ni-NTA column. SDS-PAGE gel was stained using Coomassie Brilliant Blue.

Abbreviations: SIV, swine influenza virus; NP, nucleoprotein; IPTG, isopropyl-β-D-thiogalactopyranoside; Ni-NTA, nickel–nitrilotriacetic acid.