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. 2019 Nov 29;14:9337–9349. doi: 10.2147/IJN.S218458

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© 2019 Du et al.

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Evaluation of specificity and reactivity of six representative sdAbs against SIV-NP.

Notes: (A) The sdAb of one representative member of each family extracted from IPTG-induced E. coli TG1 colonies was checked for their specific binding to the immobilized SIV-NP using an indirect ELISA. PEDV-N was used as an irrelevant protein control. (B) The reactivity of six representative sdAbs against SIV-NP was detected by indirect ELISA.

Abbreviations: sdAb, single-domain antibody; IPTG, isopropyl-β-D-thiogalactopyranoside; SIV, swine influenza virus; NP, nucleoprotein; PEDV, porcine epidemic diarrhea virus; N, nucleocapsid.