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. 2019 Nov 29;14:9337–9349. doi: 10.2147/IJN.S218458

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© 2019 Du et al.

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Expression and purification of sdAb5-BAP fusion protein.

Notes: (A) The expressed and (B) purified sdAb5-BAP fusion protein was analyzed by SDS-PAGE. (A) MW, protein molecular markers; lane 1, supernatant of bacterial lysates from IPTG-induced pET-21b-sdAb5-BAP-transformed cells in E.coli; lane 2, precipitation of bacterial lysates. (B) MW, protein molecular markers; Lane 1–4, sequential fractions from the Ni-NTA column.

Abbreviations: sdAb, single-domain antibody; BAP, biotin acceptor peptide; IPTG, isopropyl-β-D-thiogalactopyranoside.