Table 2.
Systemic and local T cell subsets.
| T cell basic subsets | ||||
| Marker | CD45+ | CD3+ | CD4+ | CD8+ |
| Gate | Singlets | CD45+ | CD3+ | CD3+ |
| Subset | Leukocytes | T cells | Th cells | Tc cells |
| Blood [% gate] | 65.7 | 20.6 | 83.1 | 14.9 |
| Synovial fluid [% gate] | 50.6 | 13.1 | 66.6 | 24.3 |
| Fold change [local/systemic] | 0.8 | 0.6 | 0.8 | 1.6 |
| Th subsets | ||||
| Marker | CCR7+/CD45RA+ | CCR7-/CD45RA- | CCR7-/CD45RA+ | |
| Gate | CD4+ | CD4+ | CD4+ | |
| Subset | Naïve | Effector memory | TEMRA | |
| Blood [% gate] | 39.3 | 8.2 | n <50 | |
| Synovial fluid [% gate] | 2.6 | 66.5 | n <50 | |
| Fold change [local/systemic] | 0.1 | 8.1 | — | |
| Tc subsets | ||||
| Marker | CCR7+/CD45RA+ | CCR7-/CD45RA- | CCR7-/CD45RA+ | |
| Gate | CD8+ | CD8+ | CD8+ | |
| Subset | Naïve | Effector memory | TEMRA | |
| Blood [% gate] | 45.5 | 12.5 | 25.0 | |
| Synovial fluid [% gate] | 17.5 | 51.2 | 5.0 | |
| Fold change [local/systemic] | 0.4 | 4.1 | 0.2 | |
| Treg subsets | ||||
| Marker | CD25+/CD45RA+ | CD25+/CD127- | CD25+/FoxP3+ | |
| Gate | CD4+ | CD4+ | CD4+ | |
| Subset | Naïve | Treg | Treg | |
| Blood [% gate] | n <50 | 4.1 | 4.5 | |
| Synovial fluid [% gate] | n <50 | 31.2 | 21.6 | |
| Fold change [local/systemic] | — | 7.8 | 4.8 |