Fig. 6.

A single high-dose TCDD injection leads to suppressed plasma insulin levels in vivo. Male C57Bl/6 mice were injected i.p. with either corn oil (vehicle) or TCDD (200 μg/kg) and euthanised either 7 or 14 days later (schematic timeline shown in [a]). BG, blood glucose; BW, body weight. (b) Body weight and (c) blood glucose were measured after a 4 h morning fast throughout the study. (d, e) Blood glucose and (f, g) plasma insulin were measured during a GTT on days 5 (d, f) and 12 (e, g). (h) On day 7 islets were isolated and glucose-stimulated insulin secretion (GSIS) was measured ex vivo after 1 h in LG (2.9 mmol/l) and 1 h in HG (16.7 mmol/l), followed by total insulin content measurement. All data are presented as mean ± SEM. Individual data points represent biological replicates from different mice. *p < 0.05, **p < 0.01 vs control; (b, c) two-way ANOVA with Šídák multiple comparisons test, (d–h) two-way repeated measures ANOVA with Šídák multiple comparisons test, (d–g) unpaired two-tailed t test for AUC