Fig. 7.

TCDD suppressed glucose-stimulated insulin secretion in male WT islets but not Cyp1a1/1a2 KO islets. Islets were isolated from male WT mice and mice with a global KO of Cyp1a1 and Cyp1a2 (Cyp1a1/1a2 KO) and treated ex vivo for 48 h with 10 nmol/l TCDD. (a) Cyp1a1 gene expression was expressed relative to WT control levels. (b) Insulin secretion was measured ex vivo after 1 h in LG (2.9 mmol/l) and 1 h in HG (16.7 mmol/l), followed by (c) total insulin content in islets. All data are presented as mean ± SEM. Individual data points represent biological replicates from different mice. *p < 0.05, **p < 0.01; (a) one-way ANOVA with Tukey’s multiple comparisons test, (b) two-way repeated measures ANOVA with Tukey’s multiple comparisons test