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. 2019 Dec 3;9:18152. doi: 10.1038/s41598-019-54665-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence images of EHTs of different genotypes. KI EHTs were transduced during casting with AAV6-S282 or -D282 at MOI of 1,000 vg/cell. After 16 days WT, KI-NT, KI-S282 and KI-D282 EHTs were fixed and co-stained with antibodies directed against α-actinin (green) and (a) total cMyBP-C (red) or (b) exogenous FLAG-tagged cMyBP-C (red). Nuclei were stained with DRAQ5 (blue). Scale bars = 20 µm. Higher magnifications (zoom-in) images for cMyBP-C/FLAG-cMyBP-C and merge are shown on the right side. Scale bars = 5 µm.