Fig. 2.

Dap12-dependent cell surface expression of mCD33. a Sequence alignment of the transmembrane segment of three murine Siglecs bearing a transmembrane lysine residue, shown in bold, along with hCD33. b Flow cytometry analysis of mCD33 expression on WT and CRISPR/Cas9-generated Dap12^−/− RAW264.7 cells (gray: isotype control; black: αmCD33). c WT and Dap12^−/− RAW264.7 cells were transiently transfected with mDap12 (red) or empty vector (black) and stained with αmCD33 (black and red) or isotype (gray). d Expression of mCD33 on WT (black), mCD33^−/− (gray), and Dap12^−/− (red) peripheral blood neutrophils (CD11b⁺Ly-6G⁺Ly-6C⁻). e Expression analysis of Dap12 and hCD33 on WT (black) and Dap12^−/− U937 cells (red); isotype is shown in gray and was at the same levels for both cell types.