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. 2019 Dec 3;2:450. doi: 10.1038/s42003-019-0698-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Transgenic expression of hCD33M in mouse microglial dampens phagocytosis. a Flow cytometry-based competitive phagocytosis gating strategy for WT (GFP⁻hCD33⁻) and hCD33M-Tg (GFP⁺hCD33M⁺) microglia. The competitive phagocytotic assay was carried out with hCD33M-Tg microglia on a mCD33^+/+ background (b–e) or mCD33^−/− background (f–i) where the non-transgenic (hCD33M⁻) microglia are WT or mCD33^−/−, respectively. Flow cytometry-based uptake of b, f dextran particles (14 independent experiments for mCD33^+/+ and 4 independent experiments for mCD33^−/−), c, g polystyrene beads (11 independent experiments and 6 independent experiments for mCD33^−/−), d, h myelin (5 independent experiments and 5 independent experiments for mCD33^−/−), and e, i aggregated Aβ_1-42 (5 independent experiments and 5 independent experiments for mCD33^−/−). For cells on a mCD33^+/+ background (b–e), a representative flow cytometry histogram is shown for each cargo and summary plots for each assay, where the average value for the hCD33 non-expressing cells was set to 100%. Statistical significance based on a paired Student’s T-test.