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. 2019 Dec 3;9:18246. doi: 10.1038/s41598-019-54747-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representation of KASP genotyping assays using SNP markers flanking the QTL for resistance to TSWV on chromosome A01. Different types of validation of SNPs within or close to the bin marker flanking the major QTL. (A,B) shows KASP assay using SNP A01_9530252; (C,D) shows KASP assay of SNP A01_9604392; (E,F) shows KASP results of SNP A01_9192862 in the RIL population. The genotyping data for each type were viewed using the software of SNPviewer (LGC Genomics). The scatter plots for the X and Y axes were representing the alleles for a marker in the RIL population. The blue and red clusters were the homozygous alleles showing polymorphism.