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. 2019 Dec 3;9:18174. doi: 10.1038/s41598-019-54754-2

Figure 2.

Figure 2

Indirubin promotes migration of keratinocytes, but not their proliferation. (A) HaCaT cells were treated without or with mitomycin C (MMC, 5 μg/mL) for 2 h, scratched, and treated with DMSO (0.1%) or indirubin (100 nM). Relative wound areas are shown (n = 18). (B) The wound area at 10 h post-wounding relative to that of (A) is shown. (C,D) HaCaT cells were treated with indirubin (1, 10, or 100 nM) for 24 h and were assessed for cell proliferation using (C) MTT assay or (D) BrdU incorporation assay (n = 6). (E) HaCaT cells were scratched and treated with DMSO (0.1%) or indirubin (100 nM) in the absence or presence of cytochalasin D (2 μM). Relative wound areas are shown (n = 18). All data are presented as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.