Figure 2.

Raman spectra obtained on S(0) particles formed in a S. kujiense culture with thiosulfate and sulfide, and in a S. kujiense spent medium experiment. The spectra were acquired on particles shown in Figure 1A (“β-culture” and “γ-culture”) and Figures 4D–I (“γ-spent media”). Spectra for α-S₈, β-S₈, and γ-S₈ standards are also shown. Differences in peak intensities between the samples and the reference spectra are due to crystal orientation effects.