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. 2019 Nov 27;10:2710. doi: 10.3389/fmicb.2019.02710

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Copyright © 2019 Cron, Henri, Chan, Macalady and Cosmidis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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STXM analyses of S(0) particles produced by S. kujiense in cultures with sulfide and thiosulfate after 5 weeks. (A–C) STXM images collected at carbon K-edge (288.2 eV). (D–F) map overlays: green areas are rich in sulfur and red areas are rich in organic carbon. (G) XANES spectra at the carbon K-edge obtained on the particles shown in panels (D–F) (dashed boxes). Vertical lines in (G) are located at 285 eV and 285.5 eV (1 s → π* transitions in aromatics), 287.44 eV (3 s → σ* transitions in aliphatics), 288.2 eV (1 s → π* transitions in amide groups of proteins), 288.5 eV (1 s → π* transitions in carboxylic groups), and 289.5 eV (1 s → σ* transitions in alcohols).