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. 2019 Nov 27;10:2745. doi: 10.3389/fimmu.2019.02745

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Copyright © 2019 Roy, Kumar, Bossier, Norouzitallab and Vanrompay.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Global changes in DNA methylation (5-mC%) in F1–F3 brine shrimp whose parental generation (F0) was treated with phloroglucinol. Global methylated DNA levels (5-mC % to total DNA) were analyzed from brine shrimp parents (A) and three successive F1, F2, and F3 generations each time from non-infected cysts (B), non-infected juveniles (C), V. parahaemolyticus-infected larvae at 12 h post challenge (D) and V. harveyi-infected larvae at 12 h post challenge (E). The MethylFlash Global DNA Methylation (5-mC) ELISA Kit (Epigentek, USA) was used. Error bars represent the standard deviation (n = 3) and significant difference (P < 0.05) in DNA methylation levels between the control and treatment groups are indicated with an asterisk (^*, **P < 0.01).