FIGURE 2.

Lower relative cDNA levels of ND1 and ENO2 in SN DA neurons from NCS-1 KO mice compared to WT. Left: qPCR-derived relative cDNA and genomic DNA levels of mitochondrially coded NADH-ubiquinone oxidoreductase chain 1 (ND1) in individual TH positive SN DA neurons from juvenile NCS-1 KO and WT mice, relative to respective tissue cDNA derived standard curves (in pg/cell, upper), and in addition normalized to specific laser-microdissected neuron sizes (in μm² × 100, lower). Note that ND1 cDNA levels reflect cDNA and also genomic DNA-derived signals, as the mitochondrially encoded ND1 gene contains no intron, but genomic ND1 levels alone are similar between WT and KO. Right: Similar qPCR-derived relative cDNA levels for the same samples as in (A) for the neuron specific enolase (ENO2). Data are given as scatter plots with mean ± SD. Significant differences are indicated according to Mann–Whitney U-tests and marked with asterisks (^∗p ≤ 0.05; ^∗∗p < 0.01). Numbers of analyzed individual SN DA neuron-derived cDNA samples (n) are given on the x-axis. All data and statistics detailed in Supplementary Figure S2 and Supplementary Table S2.