TABLE 1.
TaqMan® assay and standard curve information.
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Standard curve data |
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| Assay ID | Target gene | Genbank accession no. (NCBI) | Amplicon length [bp] | Exon boundary | Threshold | Y-Intercept | Slope | R2 | n |
| Mm00551392_m1 | Cav1.3 (Cacna1d) | NM_001083616 | 62 | 38–39 | 0.5 | 45.81 ± 0.46 | −3.39 ± 0.02 | 1.00 ± 0.00 | 3 |
| Mm00494444_m1 | Cav2.3 (Cacna1e) | NM_009782.3 | 63 | 43–44 | 0.5 | 43.19 ± 0.22 | −3.39 ± 0.04 | 1.00 ± 0.00 | 3 |
| Mm00486549_m1 | Cav3.1 (Cacna1g) | NM_009783.3 | 57 | 9–10 | 0.5 | 42.81 ± 0.28 | −3.31 ± 0.08 | 0.99 ± 0.01 | 3 |
| Mm00498538_m1 | DJ-1 (PARK7) | NM_020569.3 | 92 | 3–4 | 0.5 0.07∗ | 42.21 ± 0.24 41.85 ± 0.70∗ | −3.41 ± 0.03 −3.56 ± 0.11∗ | 1.00 ± 0.00 0.99 ± 0.01∗ | 4 3∗ |
| Mm00469062_m1 | Eno2 | NM_013509 | 76 | 7–8 | 0.5 0.06∗ | 41.53 ± 0.72 40.31 ± 0.21∗ | −3.38 ± 0.06 −3.46 ± 0.04∗ | 0.99 ± 0.00 1.00 ± 0.00∗ | 3 3∗ |
| Mm00484700_m1 | GBA1 (Gba) | NM_008094.5 | 76 | 5–6 | 0.5 | 43.53 ± 0.55 | −3.37 ± 0.07 | 0.99 ± 0.01 | 3 |
| Mm01339777_m1 | KChip3 (Kcnip3) | NM_019789.4 | 77 | 8–9 | 0.5 | 40.81 ± 0.40 | −3.39 ± 0.05 | 1.00 ± 0.00 | 4 |
| Mm00498260_m1 | Kv4.3 (Kcnd3) | NM_019931.1 | 84 | 4–5 | 0.5 | 44.67 ± 0.13 | −3.54 ± 0.01 | 1.00 ± 0.00 | 4 |
| Mm00522265_m1 | Letm1 | NM_019694.1 | 74 | 7–8 | 0.5 | 42.22 ± 0.06 | −3.37 ± 0.02 | 1.00 ± 0.00 | 3 |
| Mm01168774_m1 | MCU (Ccdc109a) | NM_001033259.4 | 92 | 4–5 | 0.5 | 43.11 ± 0.15 | −3.44 ± 0.05 | 1.00 ± 0.00 | 4 |
| Mm00519260_m1 | mNCX (Slc8b1) | NM_133221.2 | 67 | 8–9 | 0.5 | 48.76 ± 0.71 | −3.21 ± 0.16 | 0.98 ± 0.02 | 5 |
| Mm00490549_m1 | NCS-1 | NM_019681 | 73 | 3–4 | 0.07∗ | 40.89 ± 0.64∗ | −3.49 ± 0.11∗ | 1.00 ± 0.00∗ | 4∗ |
| Mm04225274_s1 | ND1 | NC_005089_ND1.0 | 81 | – | 0.5 0.07∗ | 34.60 ± 0.44 33.23 ± 0.28∗ | −3.44 ± 0.02 −3.51 ± 0.08∗ | 1.00 ± 0.00 1.00 ± 0.00∗ | 3 4∗ |
| Mm00447181_m1 | PGC-1α (Ppargc1a) | NM_008904.2 | 78 | 3–4 | 0.5 | 41.88 ± 0.11 | −3.28 ± 0.02 | 1.00 ± 0.00 | 3 |
| Mm00447331_m1 | alphaSYN (Snca) | NM_001042451.2 | 71 | 2–3 | 0.5 | 42.00 ± 0.10 | −3.34 ± 0.03 | 1.00 ± 0.00 | 4 |
| Mm00627598_m1 | UCP2 (Slc25a8) | NM_011671.5 | 89 | 2–3 | 0.5 | 41.86 ± 0.11 | −3.21 ± 0.01 | 0.99 ± 0.01 | 3 |
| Mm00494077_m1 | UCP3 (Slc25a9) | NM_009464.3 | 69 | 5–6 | 0.5 | 47.82 ± 3.42 | −3.61 ± 0.20 | 1.00 ± 0.00 | 3 |
| Mm01277267_m1 | UCP4 (Slc25a27) | NM_028711.3 | 76 | 6–7 | 0.5 0.06∗ | 45.20 ± 0.69 43.35 ± 0.33∗ | −3.55 ± 0.12 3.65 ± 0.09∗ | 1.00 ± 0.00 0.99 ± 0.01∗ | 4 3∗ |
| Mm00488302_m1 | UCP5 (Slc25a14) | NM_001290703/4.1 | 81 | 9–10 | 0.5 0.06∗ | 44.66 ± 0.10 44.21 ± 0.09∗ | −3.28 ± 0.03 −3.55 ± 0.02∗ | 0.99 ± 0.01 0.99 ± 0.00∗ | 4 3∗ |
Threshold, Y-Intercept (± SD), slope (± SD), R2 (± SD) were determined using serial dilutions over four magnitudes of cDNA as templates, derived from mouse midbrain tissue of juvenile NCS-1 WT mice (∗Cav2.3 WT-derived).