TABLE 2.
Relative mRNA levels in SN DA neurons of either NCS-1 KO or Cav2.3 KO, normalized to individual cell sizes, and to respective WT data.
|
NCS-1 WT |
NCS-1 KO |
|||||||||||||
| Mean | ± SEM | ±SD | Median | n | N | Mean | ± SEM | ±SD | Median | n | N | p-value | ||
| ND1 cDNA | 1.00 | 0.05 | 0.46 | 0.98 | 87 (88) | 18 | 0.82 | 0.04 | 0.41 | 0.75 | 93 (94) | 18 | 0.0152∗ | |
| ND1 genom. | 1.00 | 0.09 | 0.45 | 0.94 | 24 (25) | 3 | 0.98 | 0.07 | 0.37 | 0.90 | 24 (25) | 3 | 0.8182 | |
| ENO2 | 1.00 | 0.07 | 0.59 | 0.80 | 87 (88) | 18 | 0.72 | 0.05 | 0.41 | 0.65 | 84 (94) | 18 | 0.0034∗ | |
| UCP2 | 1.00 | 0.33 | 0.93 | 0.74 | 8 (15) | 4 | 0.73 | 0.53 | 1.30 | 0.04 | 6 (15) | 4 | 0.5728 | |
| UCP4 | 1.00 | 0.10 | 0.31 | 1.00 | 9 (9) | 3 | 0.44 | 0.08 | 0.25 | 0.43 | 10 (10) | 3 | 0.0003∗ | |
| UCP5 | 1.00 | 0.24 | 0.73 | 0.71 | 9 (9) | 3 | 0.42 | 0.06 | 0.18 | 0.47 | 9 (10) | 3 | 0.0142∗ | |
| MCU | 1.00 | 0.25 | 0.74 | 0.69 | 9 (10) | 3 | 0.91 | 0.26 | 0.68 | 0.69 | 7 (10) | 3 | 0.9182 | |
| LETM1 | 1.00 | 0.17 | 0.49 | 0.92 | 8 (9) | 4 | 0.68 | 0.13 | 0.40 | 0.58 | 9 (9) | 4 | 0.0927 | |
| DJ-1 | 1.00 | 0.15 | 0.52 | 0.76 | 13 (13) | 6 | 0.45 | 0.06 | 0.19 | 0.45 | 11 (14) | 6 | 0.0129∗ | |
| SNCA | 1.00 | 0.16 | 0.45 | 0.92 | 8 (9) | 3 | 0.91 | 0.15 | 0.41 | 0.70 | 7 (8) | 4 | 0.4634 | |
| PGC-1α | 1.00 | 0.22 | 0.69 | 0.82 | 10 (10) | 4 | 1.00 | 0.32 | 0.86 | 0.85 | 7 (8) | 4 | 0.8868 | |
| GBA1 | 1.00 | 0.10 | 0.31 | 1.07 | 9 (10) | 4 | 0.78 | 0.16 | 0.45 | 0.68 | 8 (8) | 4 | 0.1996 | |
| Cav1.3 | 1.00 | 0.16 | 0.48 | 0.88 | 9 (10) | 4 | 0.82 | 0.15 | 0.48 | 0.73 | 10 (10) | 3 | 0.3562 | |
| Cav2.3# | 1.00 | 0.09 | 0.39 | 0.88 | 20 (20) | 5 | 0.53 | 0.06 | 0.28 | 0.51 | 20 (20) | 4 | < 0.0001∗ | |
| Cav3.1 | 1.00 | 0.13 | 0.40 | 1.03 | 10 (10) | 4 | 0.82 | 0.16 | 0.50 | 0.56 | 10 (10) | 4 | 0.4359 | |
| Kv4.3 | 1.00 | 0.15 | 0.46 | 0.90 | 10 (10) | 3 | 1.11 | 0.29 | 0.86 | 0.84 | 9 (10) | 3 | 0.9682 | |
| KChip3 | 1.00 | 0.16 | 0.51 | 0.90 | 10 (10) | 3 | 1.06 | 0.24 | 0.75 | 0.74 | 10 (10) | 3 | 0.8534 | |
|
Cav2.3 WT |
Cav2.3 KO |
|||||||||||||
| Mean | ± SEM | ±SD | Median | n | N | Mean | ± SEM | ±SD | Median | n | N | p-value | ||
| ND1 cDNA | 1.00 | 0.04 | 0.26 | 0.96 | 42 (43) | 5 | 1.14 | 0.04 | 0.27 | 1.07 | 44 (44) | 5 | 0.0062∗ | |
| ND1 genom. | 1.00 | 0.06 | 0.22 | 1.02 | 15 (20) | 2 | 1.24 | 0.11 | 0.49 | 1.22 | 19 (20) | 3 | 0.0153∗ | |
| ENO2 | 1.00 | 0.06 | 0.36 | 0.97 | 42 (43) | 5 | 1.06 | 0.07 | 0.44 | 1.07 | 44 (44) | 5 | 0.5330 | |
| DJ-1 | 1.00 | 0.09 | 0.30 | 0.89 | 10 (10) | 3 | 0.94 | 0.16 | 0.50 | 0.85 | 10 (10) | 3 | 0.6305 | |
| UCP4 | 1.00 | 0.10 | 0.33 | 1.02 | 10 (10) | 3 | 1.28 | 0.21 | 0.67 | 1.30 | 10 (10) | 3 | 0.2799 | |
| UCP5 | 1.00 | 0.13 | 0.54 | 0.90 | 16 (16) | 4 | 1.14 | 0.12 | 0.49 | 1.04 | 16 (16) | 4 | 0.3414 | |
Data and statistics for graphs shown in Figures 2, 3A–C, 4A,B. Data are normalized to lasered cell sizes and to WT, thus mean KO-values directly display fold-differences in relation to respective WT. (n) indicates the number of detected signals, from all tested TH positive SN neuron-derived cDNA samples (given in brackets), derived from (N) mice. (#) data modified from Benkert et al. (2019). P-values according to Mann–Whitney U-tests, significant differences are marked with (∗). Note that ND1 cDNA levels reflect cDNA + genomic DNA-derived signals, as the ND1 gene contains no intron.