TABLE 3.
Relative mRNA levels in SN DA neurons of NCS-1 KO and WT, normalized to cell sizes, in respect to that of ND1 or ENO2.
|
NCS-1 WT |
NCS-1 KO |
||||||||||||||
| Mean | ± SEM | ±SD | Median | n | N | Mean | ± SEM | ±SD | Median | n | N | p-value | |||
| qPCR results, normalized to neuron size, ND1 and WT | |||||||||||||||
| UCP4 | 1.00 | 0.06 | 0.18 | 1.02 | 9 (9) | 3 | 0.60 | 0.08 | 0.24 | 0.63 | 10 (10) | 3 | 0.0003∗ | ||
| UCP5 | 1.00 | 0.14 | 0.41 | 0.82 | 9 (9) | 3 | 0.59 | 0.09 | 0.28 | 0.51 | 9 (10) | 3 | 0.0503∗ | ||
| DJ-1 | 1.00 | 0.07 | 0.24 | 0.99 | 13 (13) | 6 | 0.89 | 0.07 | 0.20 | 0.89 | 9 (14) | 6 | 0.2921 | ||
| Cav2.3 | 1.00 | 0.08 | 0.34 | 0.91 | 20 (20) | 5 | 0.56 | 0.06 | 0.25 | 0.52 | 19 (20) | 4 | < 0.0001∗ | ||
| ENO2 | 1.00 | 0.06 | 0.53 | 0.94 | 86 (88) | 18 | 0.98 | 0.05 | 0.49 | 0.98 | 87 (94) | 18 | 0.9722 | ||
| qPCR results, normalized to neuron size, ENO2 and WT | |||||||||||||||
| UCP4 | 1.00 | 0.06 | 0.17 | 1.03 | 9 (9) | 3 | 0.67 | 0.11 | 0.34 | 0.61 | 10 (10) | 3 | 0.0350∗ | ||
| UCP5 | 1.00 | 0.15 | 0.46 | 0.79 | 9 (9) | 3 | 0.77 | 0.16 | 0.49 | 0.63 | 9 (10) | 3 | 0.1615 | ||
| DJ-1 | 1.00 | 0.17 | 0.60 | 0.85 | 12 (13) | 6 | 1.35 | 0.42 | 1.26 | 0.79 | 9 (14) | 6 | 0.9170 | ||
| Cav2.3 | 1.00 | 0.11 | 0.48 | 0.78 | 20 (20) | 5 | 0.80 | 0.10 | 0.40 | 0.68 | 18 (20) | 4 | 0.1957 | ||
| ND1 | 1.00 | 0.09 | 0.79 | 0.57 | 84 (88) | 18 | 0.79 | 0.05 | 0.49 | 0.59 | 81 (94) | 18 | 0.5682 | ||
Data and statistics for graphs shown in Figure 5. Data are normalized to lasered cell sizes, to ND1 or ENO2 and to WT, thus mean KO-values directly display fold-differences in relation to respective WTs. (n) indicates the number of detected signals, from all tested TH positive SN DA neuron-derived cDNA-samples (given in brackets), derived from (N) mice. P-values according to Mann–Whitney U-tests, significant differences are marked with (∗).