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. 2019 Dec 3;4(6):e00348-19. doi: 10.1128/mSystems.00348-19

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Copyright © 2019 Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Light-fed CR mice showed different fecal microbiota structural changes compared to dark-fed mice. (a) Principal-coordinate analysis (PCoA) based on Bray-Curtis distance of all the samples collected at two time points (ZT0 and ZT12) of the day after the 4-week calorie restriction (CR) period and of days 2, 11, 19, and 26 after ad libitum feeding. (b) Top, PCoA based on Bray-Curtis distance. Bottom, heatmap of Bray-Curtis distances between each group and permutational multivariate analysis of variance (PERMANOVA; 9,999 permutations) were shown separately on the day after 4-week CR and days 2, 11, 19, and 26 AL (from left to right). *, P < 0.05; **, P < 0.01; ***, P < 0.001.