TABLE 1.
RNA-Seq life cycle samples and library preparations
| Nematode host and sample | B. malayi | wBm | A. aegypti |
|---|---|---|---|
| Gerbil | |||
| 1 dpi | Poly(A) selection | rRNA, poly(A) depletion | NAa |
| 2 dpi | Poly(A) selection | rRNA, poly(A) depletion | NA |
| 3 dpi | Poly(A) selection | rRNA, poly(A) depletion | NA |
| 4 dpi | Poly(A) selection | rRNA, poly(A) depletion | NA |
| 8 dpi | Poly(A) selection | rRNA, poly(A) depletion | NA |
| 20-dpi male | Poly(A) selection | rRNA, poly(A) depletion | NA |
| 24-dpi female | Poly(A) selection | rRNA, poly(A) depletion and wBm AgSS | NA |
| Adult male | Poly(A) selection | rRNA, poly(A) depletion | NA |
| Adult female | Poly(A) selection | rRNA, poly(A) depletion and wBm AgSS | NA |
| Embryo | Poly(A) selection | rRNA, poly(A) depletion | NA |
| Immature microfilariae | Poly(A) selection | rRNA, poly(A) depletion | NA |
| Mature microfilariae | Poly(A) selection | rRNA, poly(A) depletion | NA |
| Mosquito | |||
| 18 hpi | B. malayi AgSS | wBm AgSS | Poly(A) selection |
| 4 dpi | Poly(A) selection and B. malayi AgSS | wBm AgSS | Poly(A) selection |
| 8 dpi | Poly(A) selection and B. malayi AgSS | wBm AgSS | Poly(A) selection |
| Infective L3 | Poly(A) selection | NA | Poly(A) selection |
a
NA, not applicable.