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. 2019 Dec 3;10(6):e01856-19. doi: 10.1128/mBio.01856-19

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Copyright © 2019 Lee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — SUMO2 is overexpressed without change in status from the free form to the conjugated form in embryonic cells. (A) Western blotting of embryonic F9 and E14 cells as indicated, transduced with vectors expressing Flag-SUMO2 or Flag-SUMO2ΔGG. Flag-SUMO1 and Flag-SUMO1ΔGG cells are included for comparison. Blots were probed with anti-Flag or anti-actin antibodies as a loading control as indicated. (B) Western blot of 293T cells transduced with vectors expressing Flag-SUMO2 or Flag-SUMO2ΔGG. SUMO1ΔGG expression is shown for comparison.