FIG 1.

Structure of the autoinhibited cytoplasmic module of V. cholerae LapD. (A) Model of posttranslational LapA regulation via LapDG and c-di-GMP in P. fluorescens. (B) Canonical model for the transcriptional regulation of adhesin proteins via c-di-GMP-responsive transcription factors based on P. fluorescens and P. aeruginosa. (C) Sequence conservation between P. fluorescens LapDG and the putative V. cholerae orthologs. (D) Crystal structure of the S helix-GGDEF-EAL domain module of V. cholerae LapD in the absence of c-di-GMP. The bottom panel shows the corresponding structure of P. fluorescens LapD for comparison. The insets show the residues comprising the S helix-EAL domain interface. (E) Superposition of the cytoplasmic modules of V. cholerae and P. fluorescens LapD in their apo state. Structures were superimposed using the EAL domain as reference. Two orthogonal views are shown.