FIG 7.

The LapDG system regulates hemagglutination. Hemagglutination assays were performed with V. cholerae O1 El Tor A1552 (A) or O1 classical O395 (B) strains. Either wild-type (WT) or indicated deletion mutants were used. The reciprocal of the lowest fold dilution (hemagglutinin [HA] titer) at which cells were able to agglutinate red blood cells was recorded. The graphs represent the means and standard deviations of HA titers from at least three biological replicates. One-way ANOVA and Dunnett’s multiple-comparison test were used to compare the mean of each mutant to WT. Mean differences with a P value of ≤0.05 were deemed significant. ***, P ≤ 0.001; ****, P ≤ 0.0001.