Figure 3.

Fmr1 deletion in VGlut1-expressing glutamatergic neurons is neither sufficient nor necessary for recapitulating the AGS phenotype. A, tdTomato fluorescence in spiral ganglion neurons in the cochlea from vGlut1^Cre/+:Rosa26 ^tdTomato/+ mice indicating Cre expression in these cells. B–D, AGS data for mice derived from vGlut1^Cre/+ and Fmr1^cOFF/y cross-breeding. Deletion in cortex does not result in a change in AGS measurements compared with WT controls. E–G, Data from vGlut1^Cre/+ and Fmr1^cON/y cross-breeding. AGS measurements resulting from Cre-dependent Fmr1 expression are no different from the cON-KO control and increased compared with WT controls. N values for AGS data were as follows: cOFF = 16, 14, 18, and 14; cON = 14, 15, 19, and 17. *p < 0.05, ***p < 0.001. K-W ANOVA followed by Dunn's test.