Figure 4.

Fmr1 deletion in Hoxb1-expressing cells in the brainstem and spinal cord is neither sufficient nor necessary for recapitulating the AGS phenotype. A, A live sagittal section obtained from Hoxb1^Cre/+:Rosa26 ^tdTomato/+ mice. tdTomato fluorescence indicates Cre expression in caudal Pons and posterior into the spinal cord. B–D, AGS data for mice derived from Hoxb1^Cre/+ and Fmr1^cOFF/y cross-breeding. Deletion in cortex does not result in a change in AGS measurements compared with WT controls. E–G, Data derived from Hoxb1^Cre/+ and Fmr1^cON/y cross-breeding. AGS measurements resulting from Cre-dependent Fmr1 expression are no different from the cON-KO control and are increased compared with WT controls. N values for AGS data were as follows: cOFF = 21, 22, 26, and 19; cON = 19, 29, 22, and 21. **p < 0.01. K-W ANOVA followed by Dunn's test.