Figure 4.

Mex67 binding to the NPC is independent of Dbp5. (A and B) NuRIM quantification of Mex67-EGFP intensity at the nuclear envelope upon depletion of Dbp5 via auxin induced degradation. (A) Representative wide field fluorescence microscopy images at different times after auxin treatment. (B) Intensity of Mex67-EGFP at the nuclear envelope. Mean of three biological replicates is shown. Error bars represent SEM. At least 300 cells were analyzed per condition and replicate. (C) FRAP analysis of Mex67-EGFP dynamics at the nuclear envelope upon depletion of Dbp5 via auxin-induced degradation. (C) Representative images of FRAP experiments. (D) FRAP curves normalized to prebleach and postbleach values. Mean curves of ≥50 cells are shown. (E) Half time of recovery retrieved from fitting individual FRAP curves. The line represents the median, the box represents the 25th to 75th percentile, and the whiskers extend to the 5th to 95th percentile. Data in D and E are pooled from three biological replicates. Scale bars, 2 µm.