Figure 2.

Multiple PSPs are recruited into SGs. (A) Overlap between PS and SG proteomes. The proteome of PSs (n = 83) comprises known PSPs and proteins recruited to PS-like structures identified in this study. SG proteomes were taken from Jain et al. (2016) and Markmiller et al. (2018). Also see Table S1. (B) List of PSPs recruited into SGs (appearing in SG proteome datasets in Jain et al. [2016] and Markmiller et al. [2018]). Core PSPs, i.e., those regulating NEAT1 levels and/or PS integrity, are given in red. (C) PSP recruitment into SGs. SH-SY5Y cells were treated with NaAsO₂ for 1 h, and SGs were visualized using an anti-TIAR antibody. Intensity profiles for representative SGs are also shown. Arrowheads point to SGs weakly positive for NONO. Scale bars: main image, 10 µm; inset, 2 µm. (D) PSP recruitment into SGs formed by overexpressed G3BP1-GFP. SH-SY5Y cells expressing G3BP1-GFP were treated with NaAsO₂ for 1 h. Scale bars: main image, 10 µm; inset, 2 µm. (E) Presence of PSPs in SG cores as analyzed by Western blot. SG cores were affinity purified from SH-SY5Y cells treated with NaAsO₂ for 1 h.