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. 2019 Nov 7;218(12):3986–3997. doi: 10.1083/jcb.201812144

Figure 3.

Figure 3.

Abl2 or Abl2-557-C is sufficient to regulate MT dynamics in vitro. (A) Alexa 488–labeled tubulin (green) polymerizes onto the ends of rhodamine-labeled GMPCPP-stabilized MTs (red) bound to the coverslip via anti-rhodamine antibodies (brown). (B and C) Time-lapse shots of MT growth and kymographs of MT dynamics. (D) MT elongation rates were measured alone or with 1 µM Abl2, Abl2-557-C, Abl2-688-C, MBP, or 2 µM Abl2-557-688. (E) MT elongation rates were measured alone or with 0.5 or 1 µM Abl2, 0.5 or 1 µM Abl2-557-C, or 1 or 4 µM MBP. (F) Kymographs of MT shrinkage for tubulin alone or with 1 µM Abl2. MT shortening rates were measured alone or with 1 µM Abl2, Abl2-557-C, or MBP. (G) MT catastrophe frequencies were measured alone or with 1 µM Abl2, Abl2-557-C, MBP, or 2 µM Abl2-557-688. (H) MT catastrophe frequencies were measured alone or with 0.5, 1, or 2 µM Abl2-557-C. (I) Cumulative frequencies of individual MT growth times were plotted based on kymographs of tubulin with 1 or 2 µM Abl2-557-C. Comparisons were made to tubulin control. (J) MT growth length at catastrophe was measured alone or with 1 µM Abl2, Abl2-557-C, or MBP. Error bars are presented as mean ± SEM n ≥ 100. **, P < 0.01; ****, P < 0.0001. freq., frequency; N.S., not significant.