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. Author manuscript; available in PMC: 2020 Dec 3.
Published in final edited form as: Anal Chem. 2019 Nov 12;91(23):15248–15254. doi: 10.1021/acs.analchem.9b04257

Figure 3.

Figure 3.

Representative deuterium uptake plots for BCA and the BCA-brinzolamide complex, and the relative fractional uptake (at the 24 h exchange time point) mapped onto the BCA crystal structure (PDB 1V9E), with the position of brinzolamide adapted from HCA-brinzolamide complex (PDB 1A42). The darker the blue color on the structure, the more the HDX decreases upon ligand binding. Calculations of the Relative Fractional Uptake of deuterium, as mapped on the protein structure, were performed using the Waters DynamX software (see the Supporting Information for more details).