Figure 5.

Representative deuterium uptake plots for maltose binding protein (MBP) and the MBP-maltose complex, and the relative fractional uptake (at the 4 h exchange time point) mapped onto the MBP-maltose complex crystal structure (PDB ID 1ANF). The darker the blue color on the structure, the more the HDX decreases upon ligand binding. Calculations of the Relative Fractional Uptake of deuterium, as mapped on the protein structure, were performed using the Waters DynamX software (see the Supporting Information for more details).