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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Clin Cancer Res. 2019 Sep 12;25(23):7089–7097. doi: 10.1158/1078-0432.CCR-19-2004

Fig. 2. Classification of BRAF mutants.

Fig. 2.

(A) Mouse embryonic fibroblasts (MEFs) were transfected to express the V5-tagged, indicated BRAF proteins or the same BRAF proteins together with R509H mutation. Cells were collected to assay expression of activated pathway intermediates. (B) Expression of the indicated BRAF proteins was induced in the conditional “RAS-less” MEFs after pre-treatment with 4-hydroxytamoxifen (4-OHT) to knock out the last RAS allele. Cells were collected to assay expression of activated pathway intermediates.