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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Cancer Immunol Res. 2019 Sep 20;7(12):1984–1997. doi: 10.1158/2326-6066.CIR-19-0056

Figure 3: The regulation of HLA increase was at the transcript level.

Figure 3:

(A) Representative western blots probing for HLA-A, beta-2-microglobulin (B2M), and GAPDH (loading control) in TPC1 cells at 72hrs after RET inhibitor treatments. (B) HLA and antigen processing machinery (TAP1, TAP2 and Beta-2 microglobulin) transcript levels measured by qPCR at 48hrs after RET inhibitor treatment. (C) Western blots for HLA-A, beta-2-microglobulin (B2M), and GAPDH (loading control) and (D) RNA levels of for HLA-A, beta-2-microglobulin (B2M), and TAP-1 and TAP-2 in Karpas 299 cells and SUDHL-1 cells after ceritinib treatment. qPCR experiments were in performed in technical triplicate.