Fig. 2.

ACh-evoked Ca²⁺ release was suppressed by H₂O₂. (Ai) Pseudo-colour images (green) of Ca²⁺ signalling evoked by ACh (100 nM), and ACh (100 nM) in the presence of H₂O₂. (100 μM). Scale bar: 20 μM. (Aii) Overlaid Ca²⁺ signalling traces from ∼200 cells (shown in A) with the average shown as the black line in response to ACh and ACh+H₂O₂. Individual Ca²⁺ traces are coloured according to the magnitiude of the control (ACh) response. (Aiii) Rastergram plot of Ca²⁺ signals. Each red dot represents a Ca²⁺ peak in each cell (shown on the left axis) ACh (100 nM) left-side and ACh (100 nM) + H₂O₂ (100 μM) right-side. (Aiv) The effect of H₂O₂ varied on Ca²⁺ signals across cells. The panel shows traces of Ca²⁺ signalling from three typical cells from (Ai). The blue lines are the Ca²⁺ signals evoked by ACh (100 nM) and the red lines ACh (100 nM) + H₂O₂ (100 μM). Cell 3 was largely unaffected by H₂O₂. (B) Density plot of mean peak value of Ca²⁺ signalling from cells treated with ACh (100 nM) and ACh (100 nM) + H₂O₂ (100 μM). Individual data points have been coloured (from blue, low to red, high) according to the density (i.e. occurrence) of particular values (C) Summary of mean peak value of Ca²⁺ signalling in all cells. (D) Density plot of the frequency of Ca²⁺ signals. Individual data points have been coloured (from blue, low to red, high) according to the density (i.e. occurrence) of particular values (E) Summary of frequency of Ca²⁺ oscillations in all cells. (F) Summary of percentage of ACh-responsive cells. For all summary data (D–F) n = 6; *p < 0.05.