Fig. 5.

2-APB inhibits ACh-evoked Ca²⁺ signalling. (Ai) Pseudo colour (green) images of Ca²⁺ signals evoked by ACh (100 nM) and ACh (100 nM) with 2-APB (100 μM). Scale bar: 20 μM. (Aii) Overlaid Ca²⁺ signalling traces of ACh (100 nM; left) and ACh (100 nM) with 2-APB (100 μM; right). Individual Ca²⁺ traces are coloured according to the magnitiude of the control (ACh) response. (B) Density plot of mean peak value of Ca²⁺ signalling. Individual data points have been coloured (from blue, low to red, high) according to the density (i.e. occurrence) of particular values. (C) Summary data of the mean peak value of Ca²⁺ signalling (E) percentage of active cells and (D) number of peaks of Ca²⁺ signalling. For all summary data (D–E), n = 5, * p<0.05.