Fig. 8.

Inhibition of complex I blocked ACh-evoked Ca²⁺ signalling. (Ai) Pseudo colour (green) images of Ca²⁺ signals evoked by ACh (100 nM), ACh (100 nM) + Rotenone (2 μM) + oligomycin (6 μM) and after washout of rotenone and oligomycin. Scale bar: 20 μM. (Aii) Overlaid Ca²⁺ signalling traces, from the cells shown in Ai, evoked by ACh (100 nM), ACh (100 nM) + Rotenone (2 μM) + oligomycin (6 μM) and after washout of rotenone and oligomycin. (B) Density plot of mean peak value of Ca²⁺ signalling. Individual data points have been coloured (from blue, low to red, high) according to the density (i.e. occurrence) of particular values (C) Density plot of number of peaks of Ca²⁺ signalling. (D) Summary of the mean peak value of Ca²⁺ signals in all cells, (E) Percentage of active cells (F) and the frequency of signals in all cells. For all summary data (D–F), n = 3, *p<0.05.