Fig. 2.

Effects of IL-4 treatment, DENV infection and iminosugar treatment on IFNγ receptor and TNFα receptor expression. Monocytes isolated from the blood of multiple donors were incubated for 3 days with or without 25 ng/ml rec human IL-4, before being infected with DENV2 at a MOI of 1 for 90 min or mock-treated. Media with or without virus was aspirated from the cells and replaced with media alone, or supplemented with 100 μM NB-DNJ or 25 μM MON-DNJ. Following 2 days incubation, the cells were resuspended by scraping, fixed and stained as described in Supplemental Fig. 1 legend and analysed by flow cytometry for receptor expression. (A) Representative histogram plots of cells from a single donor for the total expression of IFNγR under the different treatments as specified in the legend. Grey filled histogram represents non-specific background as determined with cells stained with an IgG2a isotype control. The geometric mean fluorescent intensity (gMFI) of (B) total IFNγR, (C) surface IFNγR and (D) total TNFαR expression under the twelve conditions was normalised to isotype control staining. Normalised gMFI levels from cells from multiple donors are shown individually, as well as mean and SD.