Figure 5.

Cytotoxic assays testing plocabulin and quality control by Z-score analysis. A, Design of a cytotoxic assay. Four Matrigel drops containing organoids are seeded per well in MW24 plates, and triplicates are run for each drug dose (blue rectangles). D, dose. B, Plocabulin dose-responses after 4-day treatment for patient #4 in three independent assays (passages #23, #27, and #35). For all assays, Z-score >0.5. Data are mean ± SD of the triplicate runs for each independent assay (different passages). C, Representative phase-contrast images of one cytotoxic assay for patient #4 (dose-response curve in B). Scale bars, 100 μm.