Figure 1.

EST2 assay using 4-MUBu. (A) Fluorescence development of 4-MUBu by spontaneous hydrolysis in HEPES buffer (curve 1) and enzymatic hydrolysis after the addition of EST2 (curve 2). (B) Calibration curve of fluorescence intensity at increasing concentration of 4-MU in HEPES buffer for the calculation of the coefficient value necessary for the determination of the amount of reaction products from the enzymatic hydrolysis (R square value for the regression line = 0.996). The error bars represent the standard deviation (SD) from three independent experiments.